PENGGUNAAN BAP DAN TDZ UNTUK PERBANYAKAN TANAMAN GAHARU (Aquilaria malaccensis Lamk.)

Agarwood (A. malaccensis Lamk.) is one of the important tropical forest trees, which
produces a high economically valuable fragrant resinous wood. The increase of agarwood
demand from year to year leads to uncontrolled illegal harvest of this plant from its natural
habitat. To encounter this problem, there is an urgent need to develop agarwood plantation.
Tissue culture is an alternative method to provide genetically good seedlings for plantation in the
future due to its short period and mass quantity of planlet production. In addition, through this
method, its might also provide homogenous plant, and free pest and diseases. The objectives of
the study were (1) to find out the optimal concentration of BAP or TDZ for inducing shoot
multiplication of agarwood in in vitro conditions. MS (Murashige And Skoog, 1962), was used as
basal media. The experimental design of the research was completely randomized design (RAL)
with treatment of BAP concentration ( control; 0,50 ppm; 0,75 ppm; 1,0 ppm) or TDZ
concentration (control; 0,25 ppm; 0,50 ppm; 0,75 ppm), in 3 units, of replicate every units
consist of 4 bottles, every bottle containing one explants coming from axillaries and adventitious
shoot explants. Results indicated that two types of agarwood explants grown in vitro in MS basal
media containing BAP 0,50 ppm or TDZ 0,25 ppm produced the highest number of shoots and
leaves of agarwood plantlets, as well as its plantlet shoot length.