Kultivasi Mikroalga Chlorella sp. Secara Fed-Batch Menggunakan Limbah Cair Tahu Untuk Produksi Lipid

Microalga is single cell organism that can live by photosynthesize resulting
biomass and other secondary product such as protein, carbohydrate, and fat
by utilizing nutrients under appropriate environmental condition. Microalga
can be used to processing liquid organic waste in order resulting safer
effluent and easily neutralized by the nature. These study is aiming to get the
data about the effect of gradual additions of tofu liquid waste to the growth
and increase of lipid content in the microalga that cultivated in tofu liquid
waste media. Microalga were cultivated by variations in gradual media
adding. The variations are 0.45 liter in every day, 0.9 liter in every two days,
and 1.35 liter in every three days. Microalga growth were measured by an
object glass, the amount of cell density in every square field calculated using
tomacytometer by aiding of hand counter where observed under light
microscope. Total lipid content were gained by employ Bligh-dyer methode.
The results of this study show the highest specific grow rate of Chlorella sp is
0.08692/day accounted from gradual tofu liquid waste addition in every three
days. The highest lipid content is 40.879 % which gained from gradual tofu
liquid waste addition in every day. Under unfavourable condition microalga
will accumulate more lipid so that increase lipid content of it’s self. The
highest chemical oxygen demand (COD) removal is 575 mg/mL.